TAF PUBLISHING

Wound healing is a complicated, multi-step process divided into three major phases: inflammation, proliferation, and scar formation/remodeling. The compartmentalization of this process into discrete stages gives the illusion of simplicity, but in reality, it is much more complicated. For efficient healing to occur, complex interactions between multiple cell types, soluble factors, and extracellular matrix components are required to re-build the tissue; PRP is produced from the blood by centrifugation, which concentrates platelets along with several bioactive factors that can promote various aspects of tissue regeneration and protection. The rationale for using and therapeutic potential of a high concentration of platelets is based on their capacity to supply and release supraphysiologic amounts of essential growth factors and cytokines from their alpha granules to provide a regenerative stimulus that augments healing and promotes repair in tissues. Unlike platelet-rich plasma (PRP), platelet-poor plasma (PPP) does not have many platelets, but PPP has its unique healing properties. This study was an experimental study using the post-test design only control group design in an experimental laboratory. The research subjects were divided into three groups: 10 rats with incision wound at the cruris and given an injection of platelet-rich plasma, then ten white rats with incision wound at the cruris and were given an injection of platelet-poor plasma, and ten rats with incision wound at the cruris for control. The wound area was measured over seven days, the wound was harvested, and histological analysis was performed, including counting collagen finding, and will be analyzed by ANOVA test. The results showed that the amount of collagen between platelet-rich plasma and platelet-poor plasma differs significantly, with a pvalue of 0.000 (p < 0.05). The amount of collagen in the healing process of incision wounds in cruris of the rat Rattus norvegicus with the administration of PRP better than the administration of PPP. The use of platelet-rich plasma (PRP) in the process of healing fractures can be studied in humans so that it is expected to be used as a post-operative wound healing therapy.