TAF PUBLISHING

This study evaluates the antiulcer and antidiarrheal activities of Lactic Acid Bacteria (LAB) from yogurt bought in the market in Wistar rats.Three LAB strains, Lactobacillus paracasei (Y1), Lactobacillus rhamnosus (Y2), and Streptococcus thermophilus (Y3), were isolated through biochemical testing and 16S rRNA sequencing. Rats were pre-treated with LAB strains for seven days before the induction of stomach ulcers by indomethacin in the antiulcer study. Ulcer index and gastric juice parameters were investigated. Castor oil was employed to cause diarrhea for the antidiarrheal test, and LAB pre-treatment impacts on fecal output, consistency, and intestinal transit time were determined. GC-MS analysis of LAB supernatants showed bioactive chemicals accountable for the reported effects. Results indicated L. paracasei (Y1) reduced the ulcer index by 65% (p<0.05) and mucus production was enhanced by 40% in comparison to controls. L. rhamnosus (Y2) reduced the severity of diarrhea by 50% (p<0.05) and normalized the intestinal transit time. S. thermophilus (Y3) showed good improvement on both ulcer and diarrhea parameters. Bioactive compounds such as lactic acid, acetic acid, butyric acid, reuterin, and bacteriocins were identified that may be responsible for potential protective effects.These results demonstrate that LAB isolated from yogurt exhibit remarkable antiulcer and antidiarrheal activity, of which L. paracasei and L. rhamnosus with the most promising benefits. In public health interests, governments can contemplate enacting laws to require use of these probiotic strains in functional foods and conducting information campaigns to educate the public about the gastrointestinal health effects of fermented foods. Moreover, future research funding should be directed to clarify the mechanisms and possible health effects of LAB, as well as adopting quality systems for guaranteeing the efficacy of probiotic commodities in the market.