TAF PUBLISHING

A study was conducted to determine the effects of different plant growth hormones towards in vitro regeneration of a new Malaysian Rice Variety (Oryza Oryza sativa L. Var. MRIA 1. L. Var. MRIA 1). Stem explants of 4-week-old aseptic seedlings were selected, and a total of 16 different combinations of NAA, IBA, BAP, and Kn were utilized. The most suitable plant growth hormones for in vitro regeneration of Oryza Oryza sativa L. var. MRIA 1. L. Var. MRIA 1 was obtained from MS (Murashige and Skoog) media supplemented with 1.0 mg/l NAA and 0.5 mg/l Kn with an average of 8.90 ± 1.79 number of shoots, 114.00 ± 18.90 number of roots, 10.70 ± 2.62 cm length of shoots, and 8.70 ± 1.64 number of leaves per explant. It was proved that the in vitro regeneration of Oryza Oryza sativa L. var. MRIA 1. L. Var. MRIA 1 from stem explants was achieved without entering the intermediate callus phase within four weeks of culture. Thus, laborious subculturing processes were not required to regenerate the multiple shoots. These findings provided an optimum plant growth hormone concentration for the tissue culture system of Oryza Oryza sativa L. var. MRIA 1. L. Var. MRIA 1. Acclimatization of regenerated plantlets of Oryza Oryza sativa L. var. MRIA 1. L. Var. MRIA 1 is recommended to serve as a complete in vitro regeneration protocol for the variety.